Real time PCR is a technique that allows for the quantification of DNA molecules in samples. DNA amplicons are measured as an absolute value or as a relative value normalized to an endogenous control gene or DNA input. Copies are measured by either non-specific fluorescent dyes, which intercalate with double stranded DNA, such as SYBR green, or by specific DNA probes tagged with emitters, as well as quenchers.
StepOnePlus Real-Time PCR System
The StepOnePlusTM Real-Time PCR system is a 96-well Real-Time PCR instrument. This system can be setup in a variety of configurations and is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications including:
- SNP genotyping
- Gene expression analysis
- MicroRNA expression
- Protein expression
- Translocation analysis
- Gene detection
- Viral load analysis
Advanced software and instrumentation for performing a wide array of genomic assays.
- Standard curve (absolute quantitation)
- Relative standard curve
- Comparative Ct (ΔΔCt) (relative quantitation)
- Genotyping and presence⁄absence
- Melt curve analysis (as a standalone application)
- High resolution melting (as a standalone application)
Sensitive four-color optical LED recording system records data from all filters for every run. Fluorescent dyes include:
- FAM™⁄SYBR® Green
- VIC®⁄JOETM
- NED™⁄TAMRA™
- ROX™
Contact: Dr. Yutaka Amemiya, PhD
Phone: 416-480-6100 ext. 63108
E-mail: yamemiya@sri.utoronto.ca