Real time PCR is a technique that allows for the quantification of DNA molecules in samples. DNA amplicons are measured as an absolute value or as a relative value normalized to an endogenous control gene or DNA input. Copies are measured by either non-specific fluorescent dyes, which intercalate with double stranded DNA, such as SYBR green, or by specific DNA probes tagged with emitters, as well as quenchers.

StepOnePlus Real-Time PCR System

StepOnePlus Real-Time PCR System

The StepOnePlusTM Real-Time PCR system is a 96-well Real-Time PCR instrument. This system can be setup in a variety of configurations and is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications including:

  • SNP genotyping
  • Gene expression analysis
  • MicroRNA expression
  • Protein expression
  • Translocation analysis
  • Gene detection
  • Viral load analysis

Advanced software and instrumentation for performing a wide array of genomic assays.

  • Standard curve (absolute quantitation)
  • Relative standard curve
  • Comparative Ct (ΔΔCt) (relative quantitation)
  • Genotyping and presence⁄absence
  • Melt curve analysis (as a standalone application)
  • High resolution melting (as a standalone application)

Sensitive four-color optical LED recording system records data from all filters for every run. Fluorescent dyes include:

  • FAM™⁄SYBR® Green
  • VIC®⁄JOETM
  • NED™⁄TAMRA™
  • ROX

Contact: Dr. Yutaka Amemiya, PhD
Phone: 416-480-6100 ext. 63108
E-mail: yamemiya@sri.utoronto.ca