Microarray reading

ScanArray Express is a microarray scanner from PerkinElmer Life Science.

The scanner is upgradeable with wavelengths to support almost 70 fluorescing dyes. It can be used for both microarray data acquisition and data quantification. It can be run for both easy scan and scan protocol. It can check arrays for spot presence before hybridization.

The microarray protocol currently used in our facility is a two-colour system. Typically, the control RNA sample is labeled with cy3, the experimental sample is labeled with cy5 and the mixture is allowed to hybridize on a single array for comparison. Two lasers with wavelengths 543 nm (green) and 633 nm (red) are used for scanning. In simple terms, if a gene is equally expressed in control and experimental samples, then equal amount of green and red fluorophor are bound to the spot. Equal amounts of green and red produce a yellow spot. If a spot is red, in this case, the gene is expressed more by experimental sample and said to be up-regulated. Conversely, a green spot represents gene to be down-regulated.

• See price list
• Contact: Yutaka Amemiya
  Phone: 416-480-6100, ext. 3108 or 3502
  E-mail: yamemiya@sri.utoronto.ca