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A process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase.

In affinity chromatography, a macromolecule (like protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be retained on the column.

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