Microscope
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Equipment

Automated widefield microscope – EVOS FL Auto (Invitrogen)

  • 4X, 10X, 20X, 40X Long working distance air objectives
  • Light cubes with integrated filter and LED illumination for DAPI, CFP, GFP, RFP, CY5, Acridine Orange, CFP-YFP (FRET)
  • XY-automated stage with adaptors for various lab ware, including slides, flasks and plates
  • Dual monochrome CCD and colour CMOS camera

Automated widefield screening microscope – IC200 (Vala Sciences)

  • Objectives: 4X, 10X, 20X, 40X air
  • Solid-state light engine (UV-Vis-IR) and filters for DAPI, FITC, TEXASRED, CY5, CY7
  • Laser- and image-based autofocus
  • XYZ-automated stage
  • sCMOS camera

Confocal spectroscopy + imaging microscope – ALBA (ISS)

  • Objectives: 20X air, 40X oil, 60X water
  • 445, 470, 488, 514, 588, 594, 640 nm laser excitation (pulsed and CW available)
  • Nikon Eclipse Ti with XYZ-automated stage
  • Avalanche photodiode detectors
  • Environment controls for regulation of temperature and CO2

Confocal screening microscope – Opera (PerkinElmer)

  • Objectives: 20X/40X air, 20X/40X/60X water
  • 405, 488, 531, 635 nm laser excitation, and emission filters for DAPI, CFP, GFP, RFP, DRAQ5
  • Laser-based autofocus
  • XYZ-automated stage
  • CCD cameras with simultaneous multichannel image acquisition
  • Environment controls for regulation of temperature and CO2
  • Integrated plate incubator and robotic plate handler

Spectrofluorometers – PTI Quantamaster (Horiba)

  • Fianium ultra-broadband supercontinuum pulsed excitation source (VIS-NIR)
  • Xenon arc lamp (UV-IR)
  • Automated multiposition sample holder
  • Temperature controls

Microplate readers – Infinite M1000 PRO, Infinite F200 PRO, Sunrise (Tecan)

  • Microplate readersMultichannel excitation, emission, absorbance and 3-D scanning
  • Wavelength selection from UV to NIR and adjustable bandwidth
  • Temperature controls
  • Integrated plate stackers

Robotic workstation – SP-Workcell (Thermo)

  • Featuring Hamilton STARlet liquid handler with 96-tip and variable-span 8-tip heads
  • Integrated Thermo-CRS VAL 3-axis robot, bulk liquid dispensers, plate washers, plate sealer, plate storage

Fully enclosed under partial vacuum with HEPA air filtering for BSL2 requirements

Contact

Jarkko Ylanko
jarkko.ylanko@
sunnybrook.ca

416-480-6100, ext. 85484

Sunnybrook Health Sciences Centre
2075 Bayview Avenue, M7 231
Toronto, ON Canada M4N 3M5