Equipment

Automated widefield microscope – EVOS FL Auto (Invitrogen)

• 4X, 10X, 20X, 40X Long working distance air objectives
• Light cubes with integrated filter and LED illumination for DAPI, CFP, GFP, RFP, CY5, Acridine Orange, CFP-YFP (FRET)
• XY-automated stage with adaptors for various lab ware, including slides, flasks and plates
• Dual monochrome CCD and colour CMOS camera

Automated widefield screening microscope – IC200 (Vala Sciences)

• Objectives: 4X, 10X, 20X, 40X air
• Solid-state light engine (UV-Vis-IR) and filters for DAPI, FITC, TEXASRED, CY5, CY7
• Laser- and image-based autofocus
• XYZ-automated stage
• sCMOS camera

Confocal spectroscopy + imaging microscope – ALBA (ISS)

• Objectives: 20X air, 40X oil, 60X water
• 445, 470, 488, 514, 588, 594, 640 nm laser excitation (pulsed and CW available)
• Nikon Eclipse Ti with XYZ-automated stage
• Avalanche photodiode detectors
• Environment controls for regulation of temperature and CO₂

Confocal screening microscope – Opera (PerkinElmer)

• Objectives: 20X/40X air, 20X/40X/60X water
• 405, 488, 531, 635 nm laser excitation, and emission filters for DAPI, CFP, GFP, RFP, DRAQ5
• Laser-based autofocus
• XYZ-automated stage
• CCD cameras with simultaneous multichannel image acquisition
• Environment controls for regulation of temperature and CO₂
• Integrated plate incubator and robotic plate handler

Spectrofluorometers – PTI Quantamaster (Horiba)

• Fianium ultra-broadband supercontinuum pulsed excitation source (VIS-NIR)
• Xenon arc lamp (UV-IR)
• Automated multiposition sample holder
• Temperature controls

Microplate readers – Infinite M1000 PRO, Infinite F200 PRO, Sunrise (Tecan)

• Multichannel excitation, emission, absorbance and 3-D scanning
• Wavelength selection from UV to NIR and adjustable bandwidth
• Temperature controls
• Integrated plate stackers

Robotic workstation – SP-Workcell (Thermo)

• Featuring Hamilton STARlet liquid handler with 96-tip and variable-span 8-tip heads
• Integrated Thermo-CRS VAL 3-axis robot, bulk liquid dispensers, plate washers, plate sealer, plate storage

Fully enclosed under partial vacuum with HEPA air filtering for BSL2 requirements