Fees
The support for the lab comes from collaborative research projects funded by grants. In addition, we provide core services using a cost-recovery approach. Below are examples of fees we charge. Since each project is unique, a detailed estimate will be made for your project. For a customized estimate, fill in and email the order form to yulia.yerofeyeva@sri.utoronto.ca; or call 416-480-4265 or 416-480-6100 ext. 67479 to arrange a meeting.
Please note that there is an SRI-regulated overhead charge for external academic and industry collaborators. A material transfer agreement or a collaboration agreement might be required.
Tissue processing and embedding
Large tissue specimens from lumpectomy, mastectomy or prostatectomy are routinely processed and embedded in our laboratory. These services are available for a fee. Please contact the laboratory manager for a price estimate.
Immunohistochemical and special staining
Examples of special stains routinely made in the biomarker imaging research lab:
- Masson trichrome stain
- Prussian blue stain
- Picrosirius red stain
- Alizarin red stain
- Goldner's trichrome stain
- Luxol fast blue stain
- Bielschowsky stain
- Pentachrome stain
- Verhoeff-Van Gieson (VVG)
- Pimonidazole (hypoxia) stain
Examples of immune stains used:
| Antibody | Company |
|---|---|
| 14-33 | Santa cruz |
| ALDH1 | BD Transduction Laboratories |
| CD105 | Millipore |
| CD206 | Abcam |
| CD24 (SN3b) | Thermo |
| CD31 | Dako |
| CD34 | Dako |
| CD44 (156-3C11) | Thermo |
| CD45 | Dako |
| CD68 | Dako |
| CK5/6 | Millipore |
| Cox2 | Invitrogen |
| Cytokeratin (AE1/AE3) | Dako |
| D240 | Abcam |
| ER (6F11) | Novocastra |
| GFAP | |
| Her2/Neu (SP3) | Thermo |
| Ki67 | Thermo |
| Neurofilament | Millipore |
| Osteopontin | Novus Biological |
| p16(discontinued) | Millipore |
| p21 | Various |
| p53 | Various |
| PR (16) | Novocastra |
| RAM 11 | Dako |
| SMA | Sigma |
Examples of fees charged per slide for immunostaining are based on 10 slides processed in one batch. The fee varies greatly depending on order size, slide size and the antibody, detection or dye used. The ranges below are estimates only. A one-time new antibody optimization fee, if required, is about $600 for internal customers.
| Slide size | 1"x3" | 2"x3" |
3"x4” |
5"x7" |
|---|---|---|---|---|
| Internal rate (per slide) | $30–$63 | $80–$212 | $140–$375 | $180–$558 |
Scanners
Leica scanner SCN400
The Leica SCN400 slide scanner provides a fully digitized image of a specimen that can be viewed and edited from computers. Three hundred and eight-four (384) slides can be scanned automatically with one-button operation. Fee is per slide.
| User type/magnification | 1”x3” | 2”x3” | 3”x3.5” | ||
| 20X | 40X | 20X | 40X | X20 | |
| Internal rate (per slide) | $7 | $9 | $10 | $15 | $25 |
|---|---|---|---|---|---|
TissueScope scanner
The advantage of this scanner is the ability to digitize whole-mount large (up to 5”x7”) slides as well as regular 1”x3” sides (12 at a time) in bright-field and fluorescent modes. Please contact the lab for a specific quote and calendar booking.
Examples of pricing per slide (depends on amount of tissue and mode). This instrument is available for self-service with proper training. The hourly rate for use of the instrument is $50 for internal users.
| Slide size/magnification | 1”x3” | 2”x3” | 3"x4” | 5"x7" | ||||
| 10X | 20X | 40X | 10X | 20X | 40X | 20X | 20X | |
| Internal rate (per slide) | $5 | $7 | $9 | $10 | $15 | $20 | $25 | $50 |
|---|---|---|---|---|---|---|---|---|
Olympus VS120
Allows for high-resolution fluorescent scanning of slides (up to X100 and over optical resolution). The hourly rate is $50 for internal users.
Freezers/tissue banking
The biomarker imaging research lab is equipped with monitored -80oC and -20oC freezers. To inquire about space availability and fees please contact Yulia Yerofeyeva at 416-480-4265 or yulia.yerofeyeva@sri.utoronto.ca.
