Equipment
Instruments available for cell sorting:
BD Fusion
- Four-laser, 17-parameter, four-way, flow-cell sorter equipped for enhanced small-particle resolution
- 70-, 85- and 100-micron nozzles
- BSL2 enclosure for noninfectious human cells
- FACSAria
- 10-colour, fixed-alignment attenuated sorter equipped with UV 488 and 633 nm lasers
- 70-, 85- and 100-micron nozzles, variable speed, digital, auto setup and monitoring
- Aseptic preparation and aerosol management system available
Instruments available for flow cytometry:
- BD LSR II special order unit 1
- Four-laser (325, 405, 488 and 633 nm), 13-colour digital analyzer
- FACSDiva software
- BD LSR II special order unit 2
- Four-laser (405, 488, 561 and 633 nm), 15-colour digital analyzer
- FACSDiva software
Instruments available for imaging:
- Nikon A1 Laser Scanning Confocal Microscope
- Tiling, multiposition, Z stacks, advanced image-processing tools available
- Temperature and humidity control options for live cell imaging
- Zeiss Axiovert 200M
- Inverted stage, multichannel acquisition, time lapse, Z-stack
- Zeiss Z1 Axio Observer Confocal Spinning Disk Microscope
- Temperature and humidity control options for live cell imaging
- High-speed camera 50fps, humidified, heated inserts, tiling, multiposition, Z stacks, advanced image-processing tools
Contact
Dr. Kevin Conway
kevin.conway@sri.utoronto.ca
416-480-6100 ext. 67282
Sunnybrook Health Sciences Centre
2075 Bayview Avenue, M7 301
Toronto, ON Canada M4N 3M5