Centre for Flow Cytometry and Microscopy
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Equipment

Instruments available for cell sorting:

  • BD Fusion.BD Fusion
    • Four-laser, 17-parameter, four-way, flow-cell sorter equipped for enhanced small-particle resolution
    • 70-, 85- and 100-micron nozzles
    • BSL2 enclosure for noninfectious human cells
  • FACSAria
    • 10-colour, fixed-alignment attenuated sorter equipped with UV 488 and 633 nm lasers
    • 70-, 85- and 100-micron nozzles, variable speed, digital, auto setup and monitoring
    • Aseptic preparation and aerosol management system available

Instruments available for flow cytometry:

  • BD LSR II special order unit 1
    • Four-laser (325, 405, 488 and 633 nm), 13-colour digital analyzer
    • FACSDiva software
  • BD LSR II special order unit 2
    • Four-laser (405, 488, 561 and 633 nm), 15-colour digital analyzer
    • FACSDiva software

    ImagingInstruments available for imaging:

    • Nikon A1 Laser Scanning Confocal Microscope
      • Tiling, multiposition, Z stacks, advanced image-processing tools available
      • Temperature and humidity control options for live cell imaging

    • Zeiss Axiovert 200M
      • Inverted stage, multichannel acquisition, time lapse, Z-stack
    • Zeiss Z1 Axio Observer Confocal Spinning Disk Microscope
      • Temperature and humidity control options for live cell imaging 
      • High-speed camera 50fps, humidified, heated inserts, tiling, multiposition, Z stacks, advanced image-processing tools

    Contact

    Paul Oleynik
    paul.oleynik@sri.utoronto.ca
    416-480-6100, ext. 7282

    Sunnybrook Health Sciences Centre
    2075 Bayview Avenue, M7 301
    Toronto, ON Canada M4N 3M5