Centre for Flow Cytometry and Microscopy


Below is a list of relevant published research from SRI and external scientists made possible via the Centre for Flow Cytometry and Scanning Microscopy instruments and personnel since 2010:

Allan DS, Kirkham CL, Aguilar OA, Qu LC, Chen P, Fine JH, Serra P, Awong G, Gommerman JL, Zúñiga-Pflücker JC, Carlyle JR. An in vitro model of innate lymphoid cell function and differentiation. Mucosal Immunol. 2015 Mar;8(2):340–51

Stanojcic M, Chen P, Harrison RA, Wang V, Antonyshyn J, Zúñiga-Pflücker JC, Jeschke MG. Leukocyte infiltration and activation of the NLRP3 inflammasome in white adipose tissue following thermal injury. Crit Care Med. 2014 Jun;42(6):1357–64

Zarin P, Wong GW, Mohtashami M, Wiest DL, Zúñiga-Pflücker JC. Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5658–63.

Benveniste P, Serra P, Dervovic D, Herer E, Knowles G, Mohtashami M, Zúñiga-Pflücker JC. Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. Blood. 2014 Feb 20;123(8):1167–77.

Fang L, Deng Z, Shatseva T, Yang J, Peng C, Du WW, Yee AJ, Ang LC, He C, Shan SW, Yang BB. MicroRNA miR-93 promotes tumor growth and angiogenesis by targeting integrin-β8.Oncogene. 2011 Feb 17;30(7):806–21.

Lee DY, Jeyapalan Z, Fang L, Yang J, Zhang Y, Yee AY, Li M, Du WW, Shatseva T, Yang BB. Expression of versican 3'-untranslated region modulates endogenous microRNA functions. PLoS One. 2010 Oct 25;5(10):e13599.

Du WW, Yang BB, Shatseva TA, Yang BL, Deng Z, Shan SW, Lee DY, Seth A, Yee AJ. Versican G3 promotes mouse mammary tumor cell growth, migration, and metastasis by influencing EGF receptor signaling. PLoS One. 2010 Nov 5;5(11):e13828.

Jeyapalan Z, Deng Z, Shatseva T, Fang L, He C, Yang BB. Expression of CD44 3'-untranslated region regulates endogenous microRNA functions in tumorigenesis and angiogenesis. Nucleic Acids Res. 2011 Apr;39(8):3026–41.

Shatseva T, Lee DY, Deng Z, Yang BB. MicroRNA miR-199a-3p regulates cell proliferation and survival by targeting caveolin-2.J Cell Sci. 2011 Aug 15;124(Pt 16):2826–36.

Allan DS, Rybalov B, Awong G, Zúñiga-Pflücker JC, Kopcow HD, Carlyle JR, Strominger JL. TGF-β affects development and differentiation of human natural killer cell subsets. Eur J Immunol. 2010 Aug;40(8):2289–95.

Awong G, Herer E, La Motte-Mohs RN, Zúñiga-Pflücker JC. Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. BMC Immunol. 2011 Mar 23;12:22.

Mohtashami M, Shah DK, Nakase H, Kianizad K, Petrie HT, Zúñiga-Pflücker JC. Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes.J Immunol. 2010 Jul 15;185(2):867–76. Erratum in: J Immunol. 2010 Sep 15;185(6):3777–8.

Wang D, Claus CL, Rajkumar P, Braunstein M, Moore AJ, Sigvardsson M, Anderson MK. Context-dependent regulation of hematopoietic lineage choice by HEBAlt.J Immunol. 2010 Oct 1;185(7):4109–17.

Braunstein M, Anderson MK. Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt.Eur J Immunol. 2010 Nov;40(11):3173–82.

Braunstein M, Anderson MK. Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt.Eur J Immunol. 2010 Nov;40(11):3173–82.

Braunstein M, Rajkumar P, Claus CL, Vaccarelli G, Moore AJ, Wang D, Anderson MK. HEBAlt enhances the T-cell potential of fetal myeloid-biased precursors.Int Immunol. 2010 Dec;22(12):963–72.

Braunstein M, Anderson MK. HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation.Mol Cell Biol. 2011 Mar;31(5):971–82. Epub 2010 Dec 28

Fine JH, Chen P, Mesci A, Allan DS, Gasser S, Raulet DH, Carlyle JR. Chemotherapy-induced genotoxic stress promotes sensitivity to natural killer cell cytotoxicity by enabling missing-self recognition. Cancer Res. 2010 Sep 15;70(18):7102–13.

Chen P, Mesci A, Carlyle JR. CELLISA: reporter cell-based immunization and screening of hybridomas specific for cell surface antigens. Methods Mol Biol. 2011;748:209–25.

Vaags AK, Gartley CJ, Halling KB, Dobson H, Zheng Y, Foltz WD, Dick AJ, Kruth SA, Hough MR. Migration of cells from the yolk sac to hematopoietic tissues after in utero transplantation of early and mid gestation canine fetuses. Transplantation. 2011 Apr 15;91(7):723–30.

Burgess A, Ayala-Grosso CA, Ganguly M, Jordão JF, Aubert I, Hynynen K. Targeted delivery of neural stem cells to the brain using MRI-guided focused ultrasound to disrupt the blood-brain barrier. PLoS One. 2011;6(11):e27877.

Jordão JF, Ayala-Grosso CA, Markham K, Huang Y, Chopra R, McLaurin J, Hynynen K, Aubert I. Antibodies targeted to the brain with image-guided focused ultrasound reduces amyloid-beta plaque load in the TgCRND8 mouse model of Alzheimer's disease. PLoS One. 2010 May 11;5(5):e10549.

Tomic J, Lichty B, Spaner DE. Aberrant interferon-signaling is associated with aggressive chronic lymphocytic leukemia. Blood. 2011 Mar 3;117(9):2668–80.

Shi Y, Tomic J, Wen F, Shaha S, Bahlo A, Harrison R, Dennis JW, Williams R, Gross BJ, Walker S, Zuccolo J, Deans JP, Hart GW, Spaner DE. Aberrant O-GlcNAcylation characterizes chronic lymphocytic leukemia. Leukemia. 2010 Sep;24(9):1588–98.

Buckley KM, Rast JP. Characterizing immune receptors from new genome sequences. Methods Mol Biol. 2011;748:273–98.

Amoodi HA, Mick PT, Shipp DB, Friesen LM, Nedzelski JM, Chen JM, Lin VY. Results with cochlear implantation in adults with speech recognition scores exceeding current criteria. Otol Neurotol. 2012 Jan;33(1):6–12.

Silver K, Harrison RE. Measuring immune receptor mobility by fluorescence recovery after photobleaching.Methods Mol Biol. 2011;748:155–67.

Emmenegger U, Francia G, Chow A, Shaked Y, Kouri A, Man S, Kerbel RS. Tumors that acquire resistance to low-dose metronomic cyclophosphamide retain sensitivity to maximum tolerated dose cyclophosphamide. Neoplasia. 2011 Jan;13(1):40–8

Nguyen VP, Chen SH, Pizzuto K, Cantarutti A, Terminesi A, Mendonca C, Dumont DJ. 25-Hydroxycholesterol exerts both a cox-2-dependent transient proliferative effect and cox-2-independent cytotoxic effect on bovine endothelial cells in a time- and cell-type-dependent manner. J Angiogenes Res. 2010 Nov 11;2:24.

Nguyen VP, Hanna G, Rodrigues N, Pizzuto K, Yang E, Van Slyke P, Kim H, Chen SH, Dumont DJ. Differential proteomic analysis of lymphatic, venous, and arterial endothelial cells extracted from bovine mesenteric vessels. Proteomics. 2010 Apr;10(8):1658–72.

Zittermann SI, Capurro MI, Shi W, Filmus J. Soluble glypican 3 inhibits the growth of hepatocellular carcinoma in vitro and in vivo. Int J Cancer. 2010 Mar 15;126(6):1291–301.

Li F, Shi W, Capurro M, Filmus J. Glypican-5 stimulates rhabdomyosarcoma cell proliferation by activating Hedgehog signaling. J Cell Biol. 2011 Feb 21;192(4):691–704.

Ichim CV, Atkins HL, Iscove NN, Wells RA. Identification of a role for the nuclear receptor EAR-2 in the maintenance of clonogenic status within the leukemia cell hierarchy.Leukemia. 2011 Nov;25(11):1687–96.


Dr. Kevin Conway
416-480-6100 ext. 67282

Sunnybrook Health Sciences Centre
2075 Bayview Avenue, M7 301
Toronto, ON Canada M4N 3M5